报告题目：基因进化在化学中的应用
报 告 人：王江云研究员
报告时间：2018年11月7日（周三）晚上7:00
报告地点：化学楼一号会议室
邀 请 人：刘红梅教授
报告人简介：
王江云，中国科学院生物物理研究所研究员，国家杰出青年基金获得者，教育部青年长江学者。2003年获美国伊利诺伊大学厄巴纳-香槟分校博士学位，2003-2007年在The Scripps Research Institute从事博士后研究。2008年到中国科学院生物物理研究所工作，组建了以研究生物物理化学、合成生物学及化学生物学为主要方向的课题组，主要研究内容包括：1. 发展蛋白质及RNA标记的新方法，研究蛋白质及RNA的定位、结构、折叠以及相互作用。2. 研究含有金属卟啉和铜离子的蛋白质的结构功能，并在此基础上设计新的金属酶。在国际一流期刊如Nat. Chem., Nat. Chem. Bio., Nat. Commun., JACS, Angew. Chem. Int. Ed.等上发表重要学术论文；在一些重要的国际会议如International Conference on Bioinorganic chemistry, Asian Biological Inorganic Chemistry Conference, IUPAC Chemical Biology Conference等上作大会报告或邀请报告；获得第五届中国化学会-英国皇家化学会青年化学奖、第十三届中国青年科技奖。
报告简介：
Photosensitizers, which harness light energy to upgrade weak reductants to strong reductants, are pivotal components of the natural and artificial photosynthesis machineries. However, it has proved difficult to enhance and expand their functions through genetic engineering. Here we report a genetically encoded, 27 kD photosensitizer protein (PSP), which facilitates the rational design of miniature photocatalytic CO2 reducing enzymes. Visible light drives PSP efficiently into a long-lived triplet excited state (PSP*), which reacts rapidly with NADH to generate a super-reducing radical (PSP•), which is strong enough to reduce many CO­2 reducing catalysts. We determined the 3D structure of PSP• at 1.8 Å resolution by X-ray crystallography. Genetic engineering enabled the site-specific attachment of a nickel-terpyridine complex, and modular optimization of the photochemical properties of PSP, chromophore/catalytic center distance and catalytic center microenvironment, culminating in a miniature photocatalytic CO2 reducing enzyme which has a CO2/CO conversion quantum efficiency of 2.6%.
